Introduction to Imaging Mass Cytometry™ | Ron Cohn, MA

Abstract:
Changing the course of how diseases are treated and ultimately cured requires a comprehensive understanding of complex cellular phenotypes and their interrelationships in the spatial context of the tissue microenvironment. To meet this great challenge, we developed the Hyperion™ Imaging System.

For the first time, the Hyperion Imaging System enables simultaneous high multiplex interrogation using proven CyTOF® technology together with the imaging capability to facilitate Imaging Mass Cytometry™ (IMC™) applications. This allows the deep interrogation of formalin-fixed, paraffin-embedded (FFPE) tissues or frozen tissues at subcellular resolution while preserving the contextual information in tissue architecture and cellular morphology. Today researchers are using this approach to uncover new biomarkers, explore the tissue and tumor microenvironment and understand biological mechanisms and cellular interactions within the spatial, structural and contextual information of a tissue sample. This compares to just a few markers per sample that can be assessed at any one time for conventional immunohistochemistry. Researchers can also preserve their precious samples and reduce experimental variability by using this system, which eliminates dependence on serial sections or sequential staining.

The Hyperion Imaging System brings proven CyTOF technology together with imaging capability to empower simultaneous interrogation of up 37 protein markers using Imaging Mass Cytometry (IMC). Using this system, you can deeply interrogate tissues and tumors at subcellular resolution while preserving the information in tissue architecture and cellular morphology to uncover new biomarker correlations and cell interactions. With the ability to utilize up to 135 channels to detect additional parameters, the Hyperion Imaging System is ideal to meet your image analysis needs today and well into the future.

Видео Introduction to Imaging Mass Cytometry™ | Ron Cohn, MA канала Fluidigm