Flow Cytometry Introduction - Malte Paulsen (EMBL)
https://www.ibiology.org/techniques/introduction-to-flow-cytometry
This video provides an excellent introduction to flow cytometry. Dr. Malte Paulsen covers the basic principles of the technique including fluidics, optics and data display.
Talk Overview:
Dr. Malte Paulsen gives an introduction to flow cytometry with an excellent explanation of the basic principles governing the technique. He explains how fluid flow is used to focus a sample in a laser beam. Light from the laser is scattered by cells in the sample and the degree of scatter provides information about the cell’s optical density and other characteristics. In conventional flow cytometry, lasers are used primarily to excite fluorescent antibodies bound to specific cell types. A detector with different filters allows specific wavelengths to be dissected from the overall fluorescence. This signal can then be displayed in ways that provide the most information about the cell type of interest.
Speaker Biography:
Dr. Malte Paulsen has been Head of the Flow Cytometry Core Facility at EMBL since 2015. Prior to joining EMBL, Paulsen was Head of the flow cytometry facilities first at Institute for Molecular Biology (IMB) in Mainz, and later at the National Heart and Lung Institute, Imperial College, London. Paulsen received his PhD from the German Cancer Research Center and the University of Heidelberg in 2011.
Видео Flow Cytometry Introduction - Malte Paulsen (EMBL) канала iBiology Techniques
This video provides an excellent introduction to flow cytometry. Dr. Malte Paulsen covers the basic principles of the technique including fluidics, optics and data display.
Talk Overview:
Dr. Malte Paulsen gives an introduction to flow cytometry with an excellent explanation of the basic principles governing the technique. He explains how fluid flow is used to focus a sample in a laser beam. Light from the laser is scattered by cells in the sample and the degree of scatter provides information about the cell’s optical density and other characteristics. In conventional flow cytometry, lasers are used primarily to excite fluorescent antibodies bound to specific cell types. A detector with different filters allows specific wavelengths to be dissected from the overall fluorescence. This signal can then be displayed in ways that provide the most information about the cell type of interest.
Speaker Biography:
Dr. Malte Paulsen has been Head of the Flow Cytometry Core Facility at EMBL since 2015. Prior to joining EMBL, Paulsen was Head of the flow cytometry facilities first at Institute for Molecular Biology (IMB) in Mainz, and later at the National Heart and Lung Institute, Imperial College, London. Paulsen received his PhD from the German Cancer Research Center and the University of Heidelberg in 2011.
Видео Flow Cytometry Introduction - Malte Paulsen (EMBL) канала iBiology Techniques
Показать
Комментарии отсутствуют
Информация о видео
Другие видео канала
Next Generation Sequencing 1: Overview - Eric Chow (UCSF)Next Generation Sequencing 2: Illumina NGS Sample Preparation - Eric Chow (UCSF)Hidde Ploegh (Boston Children’s Hospital) 1: Immunology: The Basics of Antibody DiversityImaging Flow Cytometry: A Brief Overview - Andrew Filby (Newcastle U.)Flow Cytometry AnalysisMass Cytometry Introduction - Susanne Heck (NIHR BRC)Flow CytometryBasics of multicolorflow cytometry panel designIntro to FlowJo v10 with Jack 9.7.17Next Generation Sequencing 3: Purifying DNA Samples with Magnetic Beads - Eric Chow (UCSF)Flow Cytometry AnimationFlow Cytometry Virtual LaboratoryBioimage Analysis 2: Pre-Processing (Kevin Eliceiri)FACS - Fluorescence Activated Cell Sorting - Steffen Schmitt (DKFZ)Flow Cytometry Animationflow cytometry : basic principlesMulticolor flow cytometry – Understanding the basics [WEBINAR]Heat Pumps: the Future of Home HeatingOptimize your Flow Cytometry - Best Practices for Sample Preparation, Staining and AnalysisCompensation of a 7 color panel on the BD LSR II